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cAMP & cGMP 检测试剂盒
cAMP & cGMP ELISA Kit

cAMP & cGMP 检测试剂盒011593583977.png

 cAMP & cGMP ELISA Kit



◆原理

 

  环状核苷酸可作为G蛋白信号通道和钙离子信号通道的第二信使。

  环状AMP(cAMP)是重要的第二信使,在很多生物学效应中起作用。已知大量激素通过能将ATP转变成cAMP的腺苷酸环化酶去激活cAMP。cAMP与血管疾病,神经系统,免疫,细胞生长和分化,代谢等有关。

  环状GMP一般比cAMP的含量水平低10~100倍,鸟苷酸环化酶作用在GTP上形成cGMP。一些激素,比如乙酰胆碱,胰岛素,催产素和其他特定的化合物比如血清素,组胺会引起cGMP水平的升高。刺激鸟苷酸环化酶的化合物,比如血管舒张药硝普钠,硝化甘油,硝酸钠和一氧化氮会提高cGMP的含量。

 

  相关疾病:心脏疾病,肿瘤,疼痛,哮喘,肥胖症,药物成瘾,精神疾病。

 


特点


●   兼容性好:可检测细胞裂解液,细胞培养上清,血清,血浆,唾液,组织或者尿液样品。可检测任意物种。

●   灵敏度高:优化的乙酰化步骤可以提高样品的检测灵敏度(可提高10倍)。

●   稳定性好:批次间的产品质量表现一致。

●   稳定性好可用于高通量筛选,3小时完成检测。

●   稳定性好简单,高效的样品处理步骤。

 


案例


●   检测小鼠视网膜裂解液

●   检测斑马鱼幼虫

●   检测软骨细胞匀浆液

●   检测N. benthamiana叶子抽提物

●   检测HUVEC细胞裂解液

●   检测大鼠肝脏组织

●   检测大鼠脑部匀浆液



cAMP  

 

Species    Reactivity

Sample Type

Sensitivity

Range

Prod. No.

Size

 cAMP CompleteEIA kit 

Species independent 

Cell lysates, culture su-pernatants,    serum, saliva and tissue 

Assay Buffer: 0.30 pmol/ml(non-acetylated) 0.039 pmol/mL (acetylated)HCl: 0.39 pmol/mL (non-acetylated)0.037 pmol/mL (acetylated)

0.78 – 200 pmol/mL (non-acetylated);    0.078 – 20 pmol/mL (acetylated) 

ADI-900-163

 

96 wells

 

ADI-900-163 

 5×96  wells

 Direct cAMP EIA kit

Species independent

Cell lysates and tissue

(non-acetylated) 0.39 pmol/mL;
 (acetylated) 0.037 pmol/mL

 0.78 – 200 pmol/mL (non- acetylated); 0.078 – 20 pmol/mL  (acetylated)

ADI-900-066

 

96 wells

ADI-901-066

5×96 wells

 cAMP EIA kit

Species independent

 Culture supernatants, serum and saliva

 (non-acetylated) 0.30 pmol/mL;
(acetylated) 0.039 pmol/mL

 0.78 –- 200 pmol/mL (non-acetylated); 0.078 –20 pmol/mL (acetylated)

ADI-900-067

96 wells

ADI-901-067

5×96 wells

 


cGMP



Species    Reactivity

Sample Type

Sensitivity

Range

Prod. No.

Size

 cGMP CompleteEIA kit

Species independent 

Cell lysates, culturesupernatants, plasma,serum, saliva, tissue andurine 

Assay Buffer : 0.42 pmol/ml(non-acetylated) 0.043 pmol/mL(acetylated) HCl: 0.604 pmol/mL (non-acetylated) 0.059 pmol/mL (acetylated)

0.8 – 500 pmol/mL (non-acetylated);  0.08 – 50pmol/mL   (acetylated) 

ADI-900-164

 

96  wells

 

ADI-901-164

 5×96  wells

 Direct cGMP EIA kit

Species independent 

Cell lysates and tissue 

(non-acetylated) 0.604 pmol/mL;(acetylated) 0.025 pmol/mL

0.8-500 pmol/mL (non-acetylated);  0.08 -50 pmol/mL   (acetylated)

ADI-900-014


96  wells

 


ADI-901-014


 5×96  wells 

 cGMP EIA kit

Species independent 

 Culture supernatants,plasma, serum, salivaand urine

 (non-acetylated) 0.37 pmol/mL;(acetylated) 0.088 pmol/mL 

0.16-500 pmol/mL (non-acetylated);   0.16-100 pmol/mL  (acetylated)

ADI-900-013


96  wells

 


ADI-901-013


 5×96  wells 




Q1: 乙酰化步骤是否是必须的?

A1: 如果样品中含有的cAMP或者cGMP浓度低或者想要提高检测灵敏度的话,样品需要进行乙酰化。乙酰化的

     灵敏度大概是非乙酰化的10倍多 。如果样品中的环状核苷酸的水平未知,推荐先检测几个样品(样品不进

     行乙酰化处理)。如果检测不到的话,样品进行乙酰化处理。

 

Q2: 是否可以用Complete ELISA试剂盒检测胞质中的cAMP/cGMP?

A2: 这些是比色法的ELISA试剂盒,定量检测任意物种的细胞裂解液,细胞培养上清,血清、唾液和组织样品

     (细胞内和细胞外的样品)的AMP/cGMP。

 

Q3: 我是否可用自己的裂解buffer(lysis buffer)?

A3 当使用偶联物(conjunction)和中和试剂时,该试剂盒已经使用HCl裂解样品和稀释标准品。如果使用不

     同的buffer,并且用HCl稀释的话,反应的pH值不同(比如如果使用其他试剂,那么设计为了中和HCl的

     中和试剂不具有校对作用)。因此,为了优化结果,建议使用HCl进行细胞裂解和稀释。

 

Q4: 我样品中的cAMP/cGMP希望的浓度是多少?

A4: 样品中的cAMP/cGMP的量取决于样品的类型,来源甚至处理的方法。不幸的话,这个不是我们能够帮助

     您帮您预测的。建议使用者查找相关资料,在这些资料红,研究的是类似的样品,条件或者检测技术。

 

Q5: 用HCl处理的裂解液与哪些蛋白检测兼容?

A5: 最好先研究一下您希望使用的蛋白检测产品。比如,Pierc有不同的BCA检测试剂盒,在他们网站都有列出

     每个试剂盒兼容的试剂。我们建议直接和蛋白检测试剂的制造商或者销售商联系。他们应该可以提供相关

     答。

 

Q6: 如果我的细胞在加入0.1M HCl后仍然没有裂解,该如何处理?

A6: 在使用0.1M HCl前,加入 Triton X-100(曲拉通 X-100)能够增加细胞或者组织的裂解。在这个浓度范围

     内使用,阴离子表面活性剂不会干扰乙酰化或者检测中结合的部分。然而,在最终检测OD值时,OD值会

     一些增加。所以,含有Triton X-100的样品检测时,相应的标准品种也要添加Triton X-100。

 

Q7: 我需要使用多少样品?建议的稀释度是多少?

A7: 样品的用量和稀释度取决于样品中含有的cAMP/cGMP。建议客户可以参考相关的文章,该文章使用的是

     似的样品类型,条件和检测技术。

 

Q8: 需要使用多少细胞?

A8: 组织的重量和细胞的数目完全取决于实验的条件。建议先测一两个样品,细胞的数目大概在2~5×106

     细胞/mL HCl。根据实验结果,有些样品可能需要进一步稀释。记住,稀释母液的方法比重新开始实验要容

     些。

 

Q9: 是否可以用Direct ELISA试剂盒检测胞质的cAMP/cGMP?

A9: 该系列试剂盒可用于检测任意物种细胞或者组织裂解液的cAMP/cGMP。

 

Q10: 在用cAMP/cGMP ELISA或者cAMP/cGMP Complete ELISA试剂盒检测细胞培养上清的cAMP/cGMP

     时是否可以使用细胞培养基?

A10: 按照说明书所说,只要用含有分泌cAMP/cGMP的同样培养基稀释标准品。OD值上发生的细微变化与样品

     和标准品放置的板具有可比性。在研发这些试剂盒时,使用了cAMP/cGMP加样的含有酚红的PRMI1640

     DMEM+Glutamax,OD值略有增加,样品也同样。建议优化自己的样品稀释度,对自己样品进行加样/

     收测试,这样会得到更好的回收率和线性数据。

 

Q11: 乙酰化标准品和样品的最大结合率只有50%,为什么?

A11: 如果样品和标准品都已经乙酰化了,但是Bo标准品没有乙酰化的话,会出现上述情况。您的数据仍然是可

     用的。计算样品浓度的OD值,不计算%B/B0分析。

 

Q12: 哪些ELISA试剂盒能够检测胞质cAMP/cGMP?

A12: Direct ELISA 试剂盒可以检测细胞内的cAMP/cGMP。如果想要检测胞内和细胞外的样品,可以使用

     complete ELISA试剂盒。

 

Q13: 是否可用这些试剂盒去研究激动剂和拮抗剂?

A13: 如果样品中的cAMP/cGMP符合试盒的检测范围,那么这些试剂盒可用于检测激动剂和拮抗剂引起的变化。

     当然,使用者最好建立样品中cAMP/cGMP 水平的基本线,优化激动剂和拮抗剂的处理。

 

Q14: 用assay buffer重悬后样品可以保存多久?

A14: 样品重悬后的稳定性不能评估。建议重悬后尽快使用。建议将样品保存在-80℃。

 

Q15: 干燥样品的话,如何处理?

A15: 使用真空离心(比如SpeedVac)或者氮气或者氩气进行干燥样品。如果样品多,建议使用真空离心的方

     法。样品可以在SpeedVac 室温放置过夜。第二天干燥,但是不能保证产品的完整性(因为不是在真空环

     境中保存样品)。

     用氮气干燥样品需要在气体末端使用玻璃枪头,将枪头正好悬在含有你样品的管子内。如果使用这个方

     法,建议首先在含有石油醚的管子上操作,确定操作流程的时间。

 

Q16: 用cAMP ELISA试剂盒Assay buffer稀释的样品,是否可用cGMP ELISA 试剂盒检测?

A16: 这两个试剂盒的Assay Buffer的货号是一样的,所以产品都是一样的。如果使用试剂盒其他所有组分,样

     也没有问题。

 

Q17: 我是否可以保存乙酰化样品一段时间,晚点再测?

A17: 不。我们建议马上检测的样品才进行乙酰化处理。样品不添加试剂进行乙酰化处理时更稳定。

 

Q18: 我细胞的样品检测后数值不是很一致。我认为我的细胞可能穿孔了。

A18: 为了获得更一致的结果,我们建议检测时用裂解液彻底破坏细胞。由于细胞的厚度不一,建议用显微镜检

     一下。

 

Q19: 需要使用多少assay buffer去重悬我的血清样品?

A19: 重悬样品的assay buffer体积完全取决于具体的实验情况。我们建议1mL assay buffer重悬1mL血清,然

     进行一定的稀释。

 

Q20: 考虑到板子是用抗兔的IgG包被的,是否可用来检测兔血清的cAMP/cGMP?

A20: 该试剂盒提供的板子包被的是能够捕获cAMP/cGMP的通用抗体。试剂盒使用的山羊抗兔IgG的抗体,意味

     着含有高内源性IgG的兔样品会引起干扰,影响cAMP/cGMP的检测。如果必须检测这些样品,我们推荐在

     检测之前使用MWCO过滤或IgG去除试剂盒。请注意,我们没有测试过上述方法,也不能提供相关实验流程。

 


◆cAMP

 cAMP complete ELISA kit

 

Product Literature References

Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets: D.R. Park, et al.; PLoS One 10, e0134962 (2015), Application(s):ELISA using acetylated mouse islets, AbstractFull Text

Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid nanoparticles in INS-1 Cells: H. Jun, et al.; J. Nanomater. 2015, Article ID 753569 (2015), Application(s): ELISA using rat INS-1 cells, Full Text

Hepatic ANGPTL3 regulates adipose tissue energy homostasis: Y. Wang, et al.; PNAS 112, 11630 (2015), Application(s):ELISA using mouse tissue homogenate, AbstractFull Text

Misoprostol modulates cytokine expression through a cAMP pathway: Potential therapeutic implication for liver disease: L. Gobejishvili, et al.; Cell Immunol. 6616, 30041 (2015), Application(s): ELISA kits quantifying cell lysates, Abstract;

Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells: P.R. van Ginkel, et al.; Toxicol. Appl. Pharmacol. (2015), Application(s): ELISA using MDA-MB-231 cells, Abstract;

Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes: S.I. Kang, et al.; Biol. Pharm. Bull. 38, 552 (2015), Application(s): ELISA, AbstractFull Text

cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, AbstractFull Text

A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes: D. Ríos-Cardona, et al.; Dev. Biol. 317, 380 (2008), Application(s): EIA using xenopus cell lysates,Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011),Abstract;

 

cAMP complete ELISA kit

 

Product Literature References

Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets: D.R. Park, et al.; PLoS One 10, e0134962 (2015), Application(s):ELISA using acetylated mouse islets, AbstractFull Text

Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid nanoparticles in INS-1 Cells: H. Jun, et al.; J. Nanomater. 2015, Article ID 753569 (2015), Application(s): ELISA using rat INS-1 cells, Full Text

Hepatic ANGPTL3 regulates adipose tissue energy homostasis: Y. Wang, et al.; PNAS 112, 11630 (2015), Application(s):ELISA using mouse tissue homogenate, AbstractFull Text

Misoprostol modulates cytokine expression through a cAMP pathway: Potential therapeutic implication for liver disease: L. Gobejishvili, et al.; Cell Immunol. 6616, 30041 (2015), Application(s): ELISA kits quantifying cell lysates, Abstract;

Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells: P.R. van Ginkel, et al.; Toxicol. Appl. Pharmacol. (2015), Application(s): ELISA using MDA-MB-231 cells, Abstract;

Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes: S.I. Kang, et al.; Biol. Pharm. Bull. 38, 552 (2015), Application(s): ELISA, AbstractFull Text

cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, AbstractFull Text

A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes: D. Ríos-Cardona, et al.; Dev. Biol. 317, 380 (2008), Application(s): EIA using xenopus cell lysates,Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011),Abstract;

 

Direct cAMP ELISA kit

 

Product Literature References

Impaired enzymatic defensive activity, mitochondrial dysfunction and proteasome activation are involved in RTT cells oxidative damage: C. Cervellati, et al.; Biochim. Biophys. Acta. (2015), Application(s): ELISA using human supernatant,Abstract;

MEK Inhibitors Reverse cAMP-Mediated Anxiety in Zebrafish: P.R. Lundegaard, et al.; Chem. Biol. 15, 330 (2015),Application(s): ELISA determining cAMP concentration in zebrafish larvae, Abstract;

Temporal cAMP signaling selectivity by natural and synthetic MC4R agonists: B.M. Molden, et al.; Mol. Endocrinol. (2015),Application(s): cAMP determination by immunoassay, AbstractFull Text

The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM: F. Zhang, et al.; EMBO Rep. (2015), Application(s): ELISA, AbstractFull Text

The neuroprotective effect of lithium in cannabinoid dependence is mediated through modulation of cyclic AMP, ERK1/2 and GSK-3β phosphorylation in cerebellar granular neurons of rat: H.R. Rahimi, et al.; Iran. J. Pharm. Res. 14, (2015),Application(s): ELISA using rat supernatant, Full Text

Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties: F. Job, et al.; FEBS Open Bio 5, 579 (2015), Application(s): ELISA using cell lysates, AbstractFull Text

Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3: M. Perez-Aso, et al.; FASEB J. 28, 802 (2014), Abstract;

Cyclin-dependent kinase 5 in the ventral tegmental area regulates depression-related behaviors: P. Zhong, et al.; J. Neurosci. 34, 6352 (2014), Application(s): EIA using mouse ventral tegmental area (VTA) homogenates, AbstractFull Text

DCC is expressed in a CD166-positive subpopulation of chondrocytes in human osteoarthritic cartilage and modulates CRE activity: A.K. Bosserhoff, et al.; Int. J. Clin. Exp. Pathol. 7, 1947 (2014), Application(s): EIA using chondrocyte homogenates, AbstractFull Text

Disruption of NHE8 Expression Impairs Leydig cell function in the testes: H. Xu, et al.; Am. J. Physiol. Cell Physiol. (2014),Abstract;

Dopamine-2 receptor activation suppresses PACAP expression in gonadotrophs: S.J. Winters, et al.; Endocrinology 155, 2647 (2014), Abstract;

Global analysis of the HrpL regulon in the plant pathogen Pseudomonas syringae pv. tomato DC3000 reveals new regulon members with diverse functions: H.N. Lam, et al.; PLoS One 9, e106115 (2014), Application(s): EIA using leaf extracts from N. benthamiana, AbstractFull Text

High throughput synthetic lethality screen reveals a tumorigenic role of adenylate cyclase in fumarate hydratase-deficient cancer cells: M. Boettcher, et al.; BMC Genomics 15, 158 (2014), Application(s): EIA using human hereditary leiomyomatosis and renal cell carcinoma (HLRCC) lysates, AbstractFull Text

Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo: N. Fakhrudin, et al.; Br. J. Pharmacol. 171, 1676 (2014), Application(s): EIA using HUVECtert lysates (acetylated samples), AbstractFull Text

Lipid droplet protein LID-1 mediates ATGL-1-dependent lipolysis during fasting in Caenorhabditis elegans: J.H. Lee, et al.; Mol. Cell. Biol. 34, 4165 (2014), Abstract;

Low concentrations of metformin suppress glucose production in hepatocytes through AMP-activated protein kinase (AMPK): J. Cao, et al.; J. Biol. Chem. 289, 20435 (2014), Abstract;

Prostaglandin E2 promotes features of replicative senescence in chronically activated human CD8+ T cells: J.P. Chou, et al.; PLoS One 9, e99432 (2014), Application(s): EIA using cell culture supernatants, AbstractFull Text

Regulator of G-protein signaling 6 (RGS6) promotes anxiety and depression by attenuating serotonin-mediated activation of the 5-HT(1A) receptor-adenylyl cyclase axis: A. Stewart, et al.; FASEB J. 28, 1735 (2014), Abstract;

Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity: M. Rouse, et al.; J. Endocrinol. 223, 107 (2014), Application(s): EIA using mouse β-Min6 lysates and primary human islet lysates, Abstract;Full Text

The metabolite 5'-methylthioadenosine signals through the adenosine receptor A2B in melanoma: K. Limm, et al.; Eur J. Cancer 50, 2714 (2014), Abstract;

Three intragenic suppressors of a GTPase-deficient allele of GNAS associated with McCune-Albright syndrome: K. Turcic, et al.; J. Mol. Endocrinol. 52, 321 (2014), Abstract;

Age-dependent alterations in cAMP signaling contribute to synaptic plasticity deficits following traumatic brain injury: D.J. Titus, et al.; Neuroscience 231, 182 (2013), Application(s): EIA using rat cortex, hippocampus and thalamus homogenates, AbstractFull Text

Calcium supplementation increases circulating cholesterol by reducing its catabolism via GPER and TRPC1-dependent pathway in estrogen deficient women: S. Li, et al.; Int. J. Cardiol. 168, 2548 (2013), Application(s): ELISA using rat liver tissue, Abstract;

Does 4-tert-octylphenol affect estrogen signaling pathways in bank vole Leydig cells and tumor mouse Leydig cells in vitro?: M. Kotula-Balak, et al.; Reprod. Toxicol. 39, 6 (2013), Abstract;

Liraglutide protects against amyloid-β protein-induced impairment of spatial learning and memory in rats: W.N. Han, et al.; Neurobiol Aging 34, 576 (2013), Application(s): EIA using rat brain homogenates, Abstract;

Molecular mechanisms underlying the rapid arrhythmogenic action of bisphenol A in female rat hearts: X. Gao, et al.; Endocrinology 154, 4607 (2013), Application(s): EIA using rat myocyte homogenates, AbstractFull Text

Resveratrol prevents β-cell dedifferentiation in nonhuman primates given a high-fat/high-sugar diet: J.L. Fiori, et al.; Diabetes 62, 3500 (2013), Application(s): EIA using CHO cell supernatant, AbstractFull Text

Role of AKAP79/150 protein in b1-adrenergic receptor trafficking and signaling in mammalian cells: X. Li, et al.; J. Biol. Chem. 288, 33797 (2013), Application(s): ELISA using murine cardiomyocytes, Abstract;

Rolofylline, an adenosine A1 receptor antagonist, inhibits osteoclast differentiation as an inverse agonist: W. He, et al.; Br. J. Pharmacol. 170, 1167 (2013), Application(s): EIA using bone marrow macrophage lysates (acetylated samples),AbstractFull Text

Constitutive cholesterol-dependent endocytosis of melanocortin-4 receptor (MC4R) Is essential to maintain receptor responsiveness to α-melanocyte-stimulating hormone (α-MSH): F.K. McDaniel, et al.; J. Biol. Chem. 287, 21873 (2012),Application(s): ELISA using human neuronal cells, Abstract;

Drugs developed to treat diabetes, liraglutide and lixisenatide, cross the blood brain barrier and enhance neurogenesis: K. Junter & C. Hölscher ; BMC Neurosci. 13, 33 (2012), Application(s): ELISA analysis of mouse brain tissue, Abstract;Full Text

Liraglutide, a long-acting GLP-1 mimetic, and its metabolite attenuate inflammation after intracerebral hemorrhage: J. Hou, et al.; J. Cereb. Blood Flow Metab. 32, 2201 (2012), Application(s): ELISA analysis of mouse brain tissue, Abstract;Full Text

Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets: H.P. de Koning, et al.; J. Infect. Dis. 206, 229 (2012), Application(s): EIA using Trypanosoma lysates, AbstractFull Text

Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention: H.M. Schuller, et al.; Carcinogenesis 33, 191 (2012), Application(s): ELISA using human pancreatic cancer xenografts, Abstract;

Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase γ and phosphodiesterase 3B: A. Baragli, et al.; Biochim. Biophys. Acta 1811, 386 (2011), Application(s): EIA using rat adipocyte extracts, Abstract;

Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress: S.J. White, et al.; J. Cell. Physiol. 226, 2841 (2011), Application(s): ELISA using HUVEC, AbstractFull Text

cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase: Chen J., et al.; Reproduction 138, 759 (2009), Abstract;

Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells: P. Hardwidge, et al. ; J. Bacteriol. 191, 178 (2009), Application(s): EIA using porcine cell lysates, Abstract;

PPARγ agonists inhibit vasopressin-mediated anion transport in the MDCK-C7 cell line: C. Nofziger, et al.; Am. J. Physiol. Renal Physiol. 297, F55 (2009), Application(s): ELISA using MDCK-C7 cell lysates, AbstractFull Text

Androgens transduce the G-alpha s-mediated activation of protein kinase A in prostate cells: Y. Daaka, et al. ; Cancer Res. 68, 3225 (2008), Application(s): EIA using human cell lysates, Abstract;

beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells: J. Olefsky, et al. ; PNAS 105, 6614 (2008), Application(s): EIA using rat cell lysates, Abstract;

Cyclic Adenosine Monophosphate Regulation of Ion Transport in Porcine Vocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008), Abstract;

FMRP acts as a key messenger for dopamine modulation in the forebrain: M. Zhao, et al. ; Neuron 59, 634 (2008),Application(s): EIA using mouse cell lysates, Abstract;

Functional evidence for presence of lipid rafts in erythrocyte membranes: Gs-alpha in rafts is essential for signal transduction: Y. Takakuwa, et al. ; Am. J. Hematol. 83, 371 (2008), Application(s): EIA using human cell lysates, Abstract;

Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: H.V. Thiagaraj, et al.; Neurochem. Int. 50, 109 (2007), Application(s): EIA using human cell lysates, Abstract;

Co-occurrence of Two Partially Inactivating Polymorphisms of MC3R Is Associated With Pediatric-Onset Obesity: N. Feng, et al. ; Diabetes 54, 2663 (2005), Application(s): EIA using human cell lysates, Abstract;

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator: T. Petnicki-Ocwieja, et al. ; J. Bacteriol. 187, 649 (2005), Application(s): EIA using plant cell lysates, Abstract;

High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract;

Lysine 270 in the Third Intracellular Domain of the Oxytocin Receptor is an Important Determinant for G{alpha}q Coupling Specificity: M. Yang, et al. ; Mol. Endocrinol. 16, 814 (2002), Application(s): EIA using monkey cell lysates, Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011),Abstract;

 

 

◆cGMP

 

cGMP ELISA kit

 

Product Literature References

Quercetin Inhibits Pacemaker Potentials via Nitric Oxide/cGMP-Dependent Activation and TRPM7/ANO1 Channels in Cultured Interstitial Cells of Cajal from Mouse Small Intestine: H. Gim, et al.; Cell. Physiol. Biochem. 35, 2422 (2015),Application(s): ELISA using murine small intestine interstitial cells of Cajal (ICCs), AbstractFull Text

The Influence of Nitric Oxide on Soluble Guanylate Cyclase Regulation by Nucleotides: the Role of Pseudosymmetric Site: N. B. Surmeli, et al.; J. Biol. Chem. (2015), Application(s): ELISA, AbstractFull Text

 

cGMP complete ELISA kit

 


Product Literature References


cGMP/PKG Signaling Suppresses Inositol 1,4,5-Trisphosphate Receptor Phosphorylation and Promotes Endoplasmic Reticulum Stress in Photoreceptors of CNG Channel-Deficient Mice: H. Ma, et al.; J. Biol. Chem. (2015), Application(s):ELISA using mouse retinal lysate, AbstractFull Text

Heterozygous NPR2 mutations cause disproportionate short stature, similar to Léri-Weill dyschondrosteosis: A. Hisado-Oliva, et al.; J. Clin. Endocrinol. Metab. (2015), Application(s): ELISA using primate supernatant, AbstractFull Text

Subcellular Trafficking of Guanylyl Cyclase/Natriuretic Peptide Receptor-A with Concurrent Generation of Intracellular cGMP: I. Nabu, et al.; Biosci. Rep. (2015), Application(s): cGMP ELISA using human cells, AbstractFull Text

cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, AbstractFull Text

cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function: J. Xu, et al.; J. Neurosci. 33, 14939 (2013), Application(s): ELISA using mouse retinal lysate, AbstractFull Text

 

Direct cGMP ELISA kit


Product Literature References

Effects of Intermittent Fasting on Age-Related Changes on Na,K-ATPase Activity and Oxidative Status Induced by LPS In Rat Hippocampus: A.R. Vasconcelos, et al.; Neurobiol. Aging (2015), Application(s): ELISA using rat brain supernatant,AbstractFull Text

PCSK6-mediated corin activation is essential for normal blood pressure: S. Chen, et al.; Nat. Med. 21, 1048 (2015),Abstract;

Dephosphorylation and inactivation of NPR2 guanylyl cyclase in granulosa cells contributes to the LH-induced decrease in cGMP that causes resumption of meiosis in rat oocytes: J. Egbert, et al.; Development 141, 3594 (2013), Application(s):EIA using rat ovarian follicles, Abstract;

Nitric oxide synthase-3 promotes embryonic development of atrioventricular valves: Y. Liu, et al.; PLoS One 8, e77611 (2013), Application(s): EIA using mouse cardiac tissue, AbstractFull Text

Sestrin 2 and AMPK connect hyperglycemia to Nox4-dependent endothelial nitric oxide synthase uncoupling and matrix protein expression: A. Eid, et al.; Mol. Cell. Biol. 33, 3439 (2013), Application(s): EIA using rat glomerular mesangial cells,AbstractFull Text

β-Sitosterol oxidation products attenuate vasorelaxation by increasing reactive oxygen species and cyclooxygenase-2: C. Yang, et al.; Cardiovasc. Res. 97, 520 (2013), Application(s): ELISA using rat aortic tissue, AbstractFull Text

Chronic deficiency of nitric oxide affects hypoxia inducible factor-1α (HIF-1α) stability and migration in human endothelial cells: M.G. Cattaneo, et al.; PLoS One 6, e29680 (2011), Application(s): EIA using human cell lysate, AbstractFull Text

cGMP secreted from the tapeworm Hymenolepis diminuta is a signal molecule to the host intestine: N. Zimmerman, et al. ; J. Parasitol. 94, 771 (2008), Application(s): EIA using tapeworm culture supernatants, Abstract;

Evidence for functional heterogeneity of circulating B-type natriuretic peptide: A. Protter, et al. ; J. Am. Coll. Cardiol. 49, 1071 (2007), Application(s): EIA using human cell lysates, Abstract;

Estradiol and progesterone modulate the nitric oxide/cyclic gmp pathway in the hypothalamus of female rats and in GT1-1 cells: H.P. Chu, et al. ; Endocrine 24, 177 (2004), Application(s): EIA using rat cell lysates, Abstract;

A Novel PDZ Protein Regulates the Activity of Guanylyl Cyclase C, the Heat-stable Enterotoxin Receptor: R.O. Scott, et al. ; J. Biol. Chem. 277, 22934 (2002), Application(s): EIA using monkey cell lysates, Abstract;

The Mechanism of Ciliary Stimulation by Acetylcholine: Roles of Calcium, PKA, and PKG: O. Zagoory, et al. ; J. Gen. Physiol. 119, 329 (2002), Application(s): EIA using frog cell lysates, Abstract;

Differential nitric oxide synthase activity, cofactor availability and cGMP accumulation in the central nervous system during anaesthesia: H.F. Galley, et al. ; Br. J. Anaesth. 86, 388 (2001), Application(s): EIA using rat cell lysates, Abstract;

Mechanism underlying diuretic effect of L-NAME at a subpressor dose: M. Liang, et al. ; Ren. Physiol. 281, F414 (2001),Application(s): EIA using rat microdialysate, AbstractFull Text

The plasma membrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I: K. Schuh, et al. ; J. Cell Biol. 155, 201 (2001), Application(s): EIA using human cell lysates, Abstract;

Role of cGMP versus 20-HETE in the vasodilator response to nitric oxide in rat cerebral arteries: C.W. Sun, et al. ; Am. J. Physiol. Heart Circ. Physiol. 279, H339 (2000), Application(s): EIA using rat cell lysates, Abstract;

Increased cerebrospinal fluid cAMP levels in Alzheimer's disease: M. Martinez, et al. ; Brain Res. 846, 265 (1999),Application(s): EIA using human cerbrospinal fluid, Abstract;

 

 


产品列表
产品编号 产品名称 产品规格 产品等级 备注
ADI-900-066 Direct cAMP ELISA kit
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ADI-901-066 Direct cAMP ELISA kit
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ADI-900-067 cAMP ELISA kit
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ADI-901-067 cAMP ELISA kit
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ADI-900-163 cAMP complete ELISA kit
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96 wells -
ADI-901-163 cAMP complete ELISA kit
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ADI-900-164 cGMP complete ELISA kit
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96 wells -
ADI-901-164 cGMP complete ELISA kit
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ADI-900-013 cGMP ELISA kit
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ADI-901-013 cGMP ELISA kit
cGMP ELISA试剂盒
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ADI-900-014 Direct cGMP ELISA kit
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ADI-901-014 Direct cGMP ELISA kit
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