10 20 30 40 50 60 70 80 90 100 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 110 120 130 140 150 160 170 180 190 200 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG BalI EarI MscI SapI BspQI 210 220 230 240 250 260 270 280 290 300 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG EheI NarI KasI BbeI SfoI 310 320 330 340 350 360 370 380 390 400 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG AvrII AccI BlnI BstZ17I Bst1107I 410 420 430 440 450 460 470 480 490 500 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT AcuI 510 520 530 540 550 560 570 580 590 600 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG PvuII 610 620 630 640 650 660 670 680 690 700 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAATTCGA ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT EcoRI Csp45I BspT104I BstBI NspV 710 720 730 740 750 760 770 780 790 800 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT TAAAAGCTTG ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG MluI HindIII 810 820 830 840 850 860 870 880 890 900 TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT 910 920 930 940 950 960 970 980 990 1000 TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT ScaI 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AATACGACTC ACTATAGGCT AGCCTGGCTT GAGGCTCAGG ACGCAAATCT TGAGGATGTT CAGCGGGAGT TTTCCGGGCT GCGAGTAATT GGTGATGAGG BmtI NheI 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 ACGAGGATGG TTCGGAGGAT GGGGAATTTT CAGACCTGGA TCTGTCTGAC AGCGACCATG AAGGGGATGA GGGTGGGGGG GCTGTTGGAG GGGGCAGGAG BsgI 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 TCTGCACTCC CTGTATTCAC TGAGCGTCGT CTAATAAAGA TGTCTATTGA TCTCTTTTAG TGTGAATCAT GTCTGACGAG GGGCCAGGTA CAGGACCTGG 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AAATGGCCTA GGAGAGAAGG GAGACACATC TGGACCAGAA GGCTCCGGCG GCAGTGGACC TCAAAGAAGA GGGGGTGATA ACCATGGACG AGGACGGGGA AvrII EarI EarI BlnI 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AGAGGACGAG GACGAGGAGG CGGAAGACCA GGAGCCCCGG GCGGCTCAGG ATCAGGGCCA AGACATAGAG ATGGTGTCCG GAGACCCCAA AAACGTCCAA 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 GTTGCATTGG CTGCAAAGGG ACCCACGGTG GAACAGGAGC AGGAGCAGGA GCGGGAGGGG CAGGAGCAGG AGGGGCAGGA GCAGGAGGAG GGGCAGGAGC SanDI 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AGGAGGAGGG GCAGGAGGGG CAGGAGGGGC AGGAGGGGCA GGAGCAGGAG GAGGGGCAGG AGCAGGAGGA GGGGCAGGAG GGGCAGGAGG GGCAGGAGCA 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 GGAGGAGGGG CAGGAGCAGG AGGAGGGGCA GGAGGGGCAG GAGCAGGAGG AGGGGCAGGA GGGGCAGGAG GGGCAGGAGC AGGAGGAGGG GCAGGAGCAG 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 GAGGAGGGGC AGGAGGGGCA GGAGCAGGAG GAGGGGCAGG AGGGGCAGGA GGGGCAGGAG CAGGAGGAGG GGCAGGAGCA GGAGGGGCAG GAGGGGCAGG 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 AGGGGCAGGA GCAGGAGGGG CAGGAGCAGG AGGAGGGGCA GGAGGGGCAG GAGGGGCAGG AGCAGGAGGG GCAGGAGCAG GAGGGGCAGG AGCAGGAGGG 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 GCAGGAGCAG GAGGGGCAGG AGGGGCAGGA GCAGGAGGGG CAGGAGGGGC AGGAGCAGGA GGGGCAGGAG GGGCAGGAGC AGGAGGAGGG GCAGGAGGGG 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 CAGGAGCAGG AGGAGGGGCA GGAGGGGCAG GAGCAGGAGG GGCAGGAGGG GCAGGAGCAG GAGGGGCAGG AGGGGCAGGA GCAGGAGGGG CAGGAGGGGC 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 AGGAGCAGGA GGAGGGGCAG GAGCAGGAGG GGCAGGAGCA GGAGGTGGAG GCCGGGGTCG AGGAGGCAGT GGAGGCCGGG GTCGAGGAGG TAGTGGAGGC 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 CGGGGTCGAG GAGGTAGTGG AGGCCGCCGG GGTAGAGGAC GTGAAAGAGC CAGGGGGGGA AGTCGTGAAA GAGCCAGGGG GAGAGGTCGT GGACGTGGAG BmgBI BmgBI 2410 2420 2430 2440 2450 2460 2470 2480 2490 2500 AAAAGAGGCC CAGGAGTCCC AGTAGTCAGT CATCATCATC CGGGTCTCCA CCGCGCAGGC CCCCTCCAGG TAGAAGGCCA TTTTTCCACC CTGTAGGGGA 2510 2520 2530 2540 2550 2560 2570 2580 2590 2600 AGCCGATTAT TTTGAATACC ACCAAGAAGG TGGCCCAGAT GGTGAGCCTG ACGTGCCCCC GGGAGCGATA GAGCAGGGCC CCGCAGATGA CCCAGGAGAA XcmI BmgBI BstXI 2610 2620 2630 2640 2650 2660 2670 2680 2690 2700 GGCCCAAGCA CTGGACCCCG GGGTCAGGGT GATGGAGGCA GGCGCAAAAA AGGAGGGTGG TTTGGAAAGC ATCGTGGTCA AGGAGGTTCC AACCCGAAAT Van91I PflMI AccB7I 2710 2720 2730 2740 2750 2760 2770 2780 2790 2800 TTGAGAACAT TGCAGAAGGT TTAAGAGCTC TCCTGGCTAG GAGTCACGTA GAAAGGACTA CCGACGAAGG AACTTGGGTC GCCGGTGTGT TCGTATATGG BsrDI SacI SgrAI EcoICRI Eco53kI 2810 2820 2830 2840 2850 2860 2870 2880 2890 2900 AGGTAGTAAG ACCTCCCTTT ACAACCTAAG GCGAGGAACT GCCCTTGCTA TTCCACAATG TCGTCTTACA CCATTGAGTC GTCTCCCCTT TGGAATGGCC BsmBI 2910 2920 2930 2940 2950 2960 2970 2980 2990 3000 CCTGGACCCG GCCCACAACC TGGCCCGCTA AGGGAGTCCA TTGTCTGTTA TTTCATGGTC TTTTTACAAA CTCATATATT TGCTGAGGTT TTGAAGGATG AccB7I BbvCI Van91I PflMI 3010 3020 3030 3040 3050 3060 3070 3080 3090 3100 CGATTAAGGA CCTTGTTATG ACAAAGCCCG CTCCTACCTG CAATATCAGG GTGACTGTGT GCAGCTTTGA CGATGGAGTA GATTTGCCTC CCTGGTTTCC BsgI 3110 3120 3130 3140 3150 3160 3170 3180 3190 3200 ACCTATGGTG GAAGGGGCTG CCGCGGAGGG TGATGACGGA GATGACGGAG ATGAAGGAGG TGATGGAGAT GAGGGTGAGG AAGGGCAGGA GTGATGTAAC 3210 3220 3230 3240 3250 3260 3270 3280 3290 3300 TTGTTAGGAG ACGCCCTCAA TCGTATTAAA AGCCGTGTAT TCCCCCGCAC TAAAGAATAA ATCCCCAGTA GACATCATGC GTGCTGTTGG TGTATTTCTG BsmBI AccI BalI MscI 3310 3320 3330 3340 3350 3360 3370 3380 3390 3400 GCCATCTGTC TTGTCACCAT TTTCGTCCTC CCAACATGGG GCAATTGGGC ATACCCATGT TGTCACGTCA CTCAGCTCCG CGCTCAACAC CTTCTCGCGT MfeI BmgBI MunI 3410 3420 3430 3440 3450 3460 3470 3480 3490 3500 TGGAAAACAT TAGCGACATT TACCTGGTGA GCAATCAGAC ATGCGACGGC TTTAGCCTGG CCTCCTTAAA TTCACCTAAG AATGGGAGCA ACCAGCAGGA SexAI 3510 3520 3530 3540 3550 3560 3570 3580 3590 3600 AAAGGACAAG CAGCGAAAAT TCACGCCCCC TTGGGAGGTG GCGGCATATG CAAAGGATAG CACTCCCACT CTACTACTGG GTATCATATG CTGACTGTAT XcmI 3610 3620 3630 3640 3650 3660 3670 3680 3690 3700 ATGCATGAGG ATAGCATATG CTACCCGGAT ACAGATTAGG ATAGCATATA CTACCCAGAT ATAGATTAGG ATAGCATATG CTACCCAGAT ATAGATTAGG EcoT22I BciVI NsiI 3710 3720 3730 3740 3750 3760 3770 3780 3790 3800 ATAGCCTATG CTACCCAGAT ATAAATTAGG ATAGCATATA CTACCCAGAT ATAGATTAGG ATAGCATATG CTACCCAGAT ATAGATTAGG ATAGCCTATG 3810 3820 3830 3840 3850 3860 3870 3880 3890 3900 CTACCCAGAT ATAGATTAGG ATAGCATATG CTACCCAGAT ATAGATTAGG ATAGCATATG CTATCCAGAT ATTTGGGTAG TATATGCTAC CCAGATATAA BstXI 3910 3920 3930 3940 3950 3960 3970 3980 3990 4000 ATTAGGATAG CATATACTAC CCTAATCTCT ATTAGGATAG CATATGCTAC CCGGATACAG ATTAGGATAG CATATACTAC CCAGATATAG ATTAGGATAG BciVI 4010 4020 4030 4040 4050 4060 4070 4080 4090 4100 CATATGCTAC CCAGATATAG ATTAGGATAG CCTATGCTAC CCAGATATAA ATTAGGATAG CATATACTAC CCAGATATAG ATTAGGATAG CATATGCTAC 4110 4120 4130 4140 4150 4160 4170 4180 4190 4200 CCAGATATAG ATTAGGATAG CCTATGCTAC CCAGATATAG ATTAGGATAG CATATGCTAT CCAGATATTT GGGTAGTATA TGCTACCCAT GGCAACATTA BstXI 4210 4220 4230 4240 4250 4260 4270 4280 4290 4300 GCCCACCGTG CTCTCAGCGA CCTCGTGAAT ATGAGGACCA ACAACCCTGT GCTTGGCGCT CAGGCGCAAG TGTGTGTAAT TTGTCCTCCA GATCGCAGCA BssSI 4310 4320 4330 4340 4350 4360 4370 4380 4390 4400 ATCGCGCCCC TATCTTGGCC CGCCCACCTA CTTATGCAGG TATTCCCCGG GGTGCCATTA GTGGTTTTGT GGGCAAGTGG TTTGACCGCA GTGGTTAGCG 4410 4420 4430 4440 4450 4460 4470 4480 4490 4500 GGGTTACAAT CAGCCAAGTT ATTACACCCT TATTTTACAG TCCAAAACCG CAGGGCGGCG TGTGGGGGCT GACGCGATCG CTGTTCCTTA GGACCCTTTT SgfI AsiSI PvuI 4510 4520 4530 4540 4550 4560 4570 4580 4590 4600 ACTAACCCTA ATTCGATAGC ATATGCTTCC CGTTGGGTAA CATATGCTAT TGAATTAGGG TTAGTCTGGA TAGTATATAC TACTACCCGG GAAGCATATG 4610 4620 4630 4640 4650 4660 4670 4680 4690 4700 CTACCCGTTT AGGGTTAACA AGGGGGCCTT ATAAACACTA TTGCTAATGC CCTCTTGAGG GTCCGCTTAT CGGTAGCTAC ACAGGCCCCT CTGATTGACG HpaI PsiI 4710 4720 4730 4740 4750 4760 4770 4780 4790 4800 TTGGTGTAGC CTCCCGTAGT CTTCCTGGGC CCCTGGGAGG TACATGTCCC CCAGCATTGG TGTAAGAGCT TCAGCCAAGA GTTACACATA AAGGCAATGT PciI AcuI BsrDI 4810 4820 4830 4840 4850 4860 4870 4880 4890 4900 TGTGTTGCAG TCCACAGACT GCAAAGTCTG CTCCAGGATG AAAGCCACTC AGTGTTGGCA AATGTGCACA TCCATTTATA AGGATGTCAA CTACAGTCAG DraIII ApaLI PsiI Alw44I 4910 4920 4930 4940 4950 4960 4970 4980 4990 5000 AGAACCCCTT TGTGTTTGGT CCCCCCCCGT GTCACATGTG GAACAGGGCC CAGTTGGCAA GTTGTACCAA CCAACTGAAG GGATTACATG CACTGCCCCA PciI AcuI BcgI 5010 5020 5030 5040 5050 5060 5070 5080 5090 5100 GCGAAGAAGG GGCAGAGATG TCGTAGTCAG GTTTAGTTCG TCCGGGGCGG GGATCTCGAC ATTGATTATT GACTAGTTAT TAATARTAAT CAATTACGGG SpeI AseI PshBI VspI 5110 5120 5130 5140 5150 5160 5170 5180 5190 5200 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA AatII ZraI 5210 5220 5230 5240 5250 5260 5270 5280 5290 5300 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GACTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG AatII ZraI 5310 5320 5330 5340 5350 5360 5370 5380 5390 5400 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG AatII ZraI 5410 5420 5430 5440 5450 5460 5470 5480 5490 5500 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT BtgZI SnaBI Eco105I 5510 5520 5530 5540 5550 5560 5570 5580 5590 5600 TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG BsgI BtgZI 5610 5620 5630 5640 5650 5660 5670 5680 5690 5700 GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG 5710 5720 5730 5740 5750 5760 5770 5780 5790 5800 AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA 5810 5820 5830 5840 5850 5860 5870 5880 5890 5900 CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC AfeI Eco47III Aor51HI 5910 5920 5930 5940 5950 5960 5970 5980 5990 6000 TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT CCGCGCTGCC 6010 6020 6030 6040 6050 6060 6070 6080 6090 6100 CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA GTGTGCGCGA GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG Eco47III Aor51HI AfeI 6110 6120 6130 6140 6150 6160 6170 6180 6190 6200 GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT CGGTCGGGCT GCAACCCCCC CTGCACCCCC BsgI 6210 6220 6230 6240 6250 6260 6270 6280 6290 6300 CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT CGGGTGCGGG GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC GGCAGGTGGG Bpu1102I BsiWI BlpI 6310 6320 6330 6340 6350 6360 6370 6380 6390 6400 GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG GGCGCGGCGG CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC SgrAI NgoMIV NaeI 6410 6420 6430 6440 6450 6460 6470 6480 6490 6500 AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC BsrDI SfoI KasI BbeI NarI EheI 6510 6520 6530 6540 6550 6560 6570 6580 6590 6600 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG KasI BbeI SfoI EheI NarI NgoMIV NaeI 6610 6620 6630 6640 6650 6660 6670 6680 6690 6700 CCTCGGGGCT GCCGCGGGGG GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG CCTCTGCTAA XbaI 6710 6720 6730 6740 6750 6760 6770 6780 6790 6800 CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTAACCCT CACTAAAGGG Acc65I KpnI 6810 6820 6830 6840 6850 6860 6870 6880 6890 6900 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCCTGCAGC CCGGGGGATC CACTAGTTCT AGAGCGGCCG CGACTCTAGA PspXI ClaI HindIIIEcoRV EcoRI BamHI SpeI XbaI NotI XbaI PaeR7I AccI BanIII BsaBI TliI SalI BspDI XhoI 6910 6920 6930 6940 6950 6960 6970 6980 6990 7000 TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT DraI BsaMI BsmI MfeI MunI 7010 7020 7030 7040 7050 7060 7070 7080 7090 7100 TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT HpaI PsiI BsaMI BsmI 7110 7120 7130 7140 7150 7160 7170 7180 7190 7200 TTGTCCAAAC TCATCAATGT ATCTTAAGGC GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC SspI 7210 7220 7230 7240 7250 7260 7270 7280 7290 7300 CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA PsiI 7310 7320 7330 7340 7350 7360 7370 7380 7390 7400 ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG BtgZI DraIII 7410 7420 7430 7440 7450 7460 7470 7480 7490 7500 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA NgoMIV NaeI 7510 7520 7530 7540 7550 7560 7570 7580 7590 7600 GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT 7610 7620 7630 7640 7650 7660 7670 7680 7690 7700 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA BciVI SspI BspHI 7710 7720 7730 7740 7750 7760 7770 7780 7790 7800 ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA EarI PvuII 7810 7820 7830 7840 7850 7860 7870 7880 7890 7900 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC SphI SexAI SphI BbuI BbuI NsiI NsiI EcoT22I EcoT22I 7910 7920 7930 7940 7950 7960 7970 7980 7990 8000 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA NmeAIII 8010 8020 8030 8040 8050 8060 8070 8080 8090 8100 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCTCGAT CGAGGGGCTC StuI PvuI NmeAIII AatI SfiI BlnI AvrII 8110 8120 8130 8140 8150 8160 8170 8180 8190 8200 GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC EcoNI CspCI 8210 8220 8230 8240 8250 8260 8270 8280 8290 8300 GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC DraI NgoMIV NaeI 8310 8320 8330 8340 8350 8360 8370 8380 8390 8400 CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTCT GAAAAACCAG AAAGTTAACT GGTAAGTTTA HpaI 8410 8420 8430 8440 8450 8460 8470 8480 8490 8500 GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA BamHI AatI StuI 8510 8520 8530 8540 8550 8560 8570 8580 8590 8600 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC EcoRI FbaI BbrPI BlnI BclI PmaCI AvrII PmlI 8610 8620 8630 8640 8650 8660 8670 8680 8690 8700 AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCGCGCA TGAAAAAGCC TGAACTCACC GCGACGTCTG PshBI ZraI VspI PshAI AseI AatII 8710 8720 8730 8740 8750 8760 8770 8780 8790 8800 TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG PflFI BssSI Tth111I BsmBI 8810 8820 8830 8840 8850 8860 8870 8880 8890 8900 TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG 8910 8920 8930 8940 8950 8960 8970 8980 8990 9000 CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG EcoRI ApaLI Alw44I DraIII 9010 9020 9030 9040 9050 9060 9070 9080 9090 9100 TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA AsiSI CspI SgfI RsrII PvuI CpoI 9110 9120 9130 9140 9150 9160 9170 9180 9190 9200 CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT PflFI Tth111I 9210 9220 9230 9240 9250 9260 9270 9280 9290 9300 CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA NmeAIII BssSI DraIII Alw44I ApaLI 9310 9320 9330 9340 9350 9360 9370 9380 9390 9400 TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA BtgZI 9410 9420 9430 9440 9450 9460 9470 9480 9490 9500 GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC 9510 9520 9530 9540 9550 9560 9570 9580 9590 9600 TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC 9610 9620 9630 9640 9650 9660 9670 9680 9690 9700 GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG GATCGGGAGA TGGGGGAGGC ScaI 9710 9720 9730 9740 9750 9760 9770 9780 9790 9800 TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCATAA 9810 9820 9830 9840 9850 9860 9870 9880 9890 9900 ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC 9910 9920 9930 9940 9950 9960 9970 9980 9990 10000 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCACTGGCC CCGTGGGTTA GGGACGGGGT CCACTAGCTA XcmI PflFI Tth111I 10010 10020 10030 10040 10050 10060 10070 10080 10090 10100 GTTCTAGTAT GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC BbuI HindIII SphI 10110 10120 10130 10140 10150 10160 10170 10180 10190 10200 ATCCCCCTTT CGCCAGGGGC GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG NarI DraI DraI SfoI KasI BbeI EheI 10210 10220 10230 10240 10250 10260 10270 10280 10290 10300 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG BspHI 10310 10320 10330 10340 10350 10360 10370 10380 10390 10400 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC AcuI 10410 10420 10430 10440 10450 10460 10470 10480 10490 10500 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 10510 10520 10530 10540 10550 10560 10570 10580 10590 10600 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 10610 10620 10630 10640 10650 10660 10670 10680 10690 10700 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC Alw44I ApaLI 10710 10720 10730 10740 10750 10760 10770 10780 10790 10800 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT BciVI BssSI 10810 10820 10830 10840 10850 10860 10870 10880 10890 10900 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 10910 10920 10930 10940 10950 10960 10970 10980 10990 11000 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCATAG PciI EcoT22BglII NsiI 11010 11020 11030 11040 11050 11060 11070 11080 11090 11100 ATCT