10 20 30 40 50 60 70 80 90 100 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 110 120 130 140 150 160 170 180 190 200 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG BalI SapI MscI BspQI 210 220 230 240 250 260 270 280 290 300 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG NarI SacII KasI BbeI EheI SfoI 310 320 330 340 350 360 370 380 390 400 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG BlnI BstZ17I AvrII AccI Bst1107I 410 420 430 440 450 460 470 480 490 500 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT BsaXI PstI 510 520 530 540 550 560 570 580 590 600 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG PvuII 610 620 630 640 650 660 670 680 690 700 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAATTCGA ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT EcoRI BspT104I BstBI Csp45I NspV 710 720 730 740 750 760 770 780 790 800 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT TAAAAGCTTG ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG AflIII PstI HindIII PstI MluI 810 820 830 840 850 860 870 880 890 900 TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT 910 920 930 940 950 960 970 980 990 1000 TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT ScaI 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AATACGACTC ACTATAGGCT AGCCTGGCTT GAGGCTCAGG ACGCAAATCT TGAGGATGTT CAGCGGGAGT TTTCCGGGCT GCGAGTAATT GGTGATGAGG NheI BmtI 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 ACGAGGATGG TTCGGAGGAT GGGGAATTTT CAGACCTGGA TCTGTCTGAC AGCGACCATG AAGGGGATGA GGGTGGGGGG GCTGTTGGAG GGGGCAGGAG BsgI 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 TCTGCACTCC CTGTATTCAC TGAGCGTCGT CTAATAAAGA TGTCTATTGA TCTCTTTTAG TGTGAATCAT GTCTGACGAG GGGCCAGGTA CAGGACCTGG 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AAATGGCCTA GGAGAGAAGG GAGACACATC TGGACCAGAA GGCTCCGGCG GCAGTGGACC TCAAAGAAGA GGGGGTGATA ACCATGGACG AGGACGGGGA BlnI AvrII 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AGAGGACGAG GACGAGGAGG CGGAAGACCA GGAGCCCCGG GCGGCTCAGG ATCAGGGCCA AGACATAGAG ATGGTGTCCG GAGACCCCAA AAACGTCCAA Aor13HI AccIII MroI BspEI 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 GTTGCATTGG CTGCAAAGGG ACCCACGGTG GAACAGGAGC AGGAGCAGGA GCGGGAGGGG CAGGAGCAGG AGGGGCAGGA GCAGGAGGAG GGGCAGGAGC SanDI 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AGGAGGAGGG GCAGGAGGGG CAGGAGGGGC AGGAGGGGCA GGAGCAGGAG GAGGGGCAGG AGCAGGAGGA GGGGCAGGAG GGGCAGGAGG GGCAGGAGCA 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 GGAGGAGGGG CAGGAGCAGG AGGAGGGGCA GGAGGGGCAG GAGCAGGAGG AGGGGCAGGA GGGGCAGGAG GGGCAGGAGC AGGAGGAGGG GCAGGAGCAG 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 GAGGAGGGGC AGGAGGGGCA GGAGCAGGAG GAGGGGCAGG AGGGGCAGGA GGGGCAGGAG CAGGAGGAGG GGCAGGAGCA GGAGGGGCAG GAGGGGCAGG 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 AGGGGCAGGA GCAGGAGGGG CAGGAGCAGG AGGAGGGGCA GGAGGGGCAG GAGGGGCAGG AGCAGGAGGG GCAGGAGCAG GAGGGGCAGG AGCAGGAGGG 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 GCAGGAGCAG GAGGGGCAGG AGGGGCAGGA GCAGGAGGGG CAGGAGGGGC AGGAGCAGGA GGGGCAGGAG GGGCAGGAGC AGGAGGAGGG GCAGGAGGGG 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 CAGGAGCAGG AGGAGGGGCA GGAGGGGCAG GAGCAGGAGG GGCAGGAGGG GCAGGAGCAG GAGGGGCAGG AGGGGCAGGA GCAGGAGGGG CAGGAGGGGC 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 AGGAGCAGGA GGAGGGGCAG GAGCAGGAGG GGCAGGAGCA GGAGGTGGAG GCCGGGGTCG AGGAGGCAGT GGAGGCCGGG GTCGAGGAGG TAGTGGAGGC 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 CGGGGTCGAG GAGGTAGTGG AGGCCGCCGG GGTAGAGGAC GTGAAAGAGC CAGGGGGGGA AGTCGTGAAA GAGCCAGGGG GAGAGGTCGT GGACGTGGAG BmgBI BsaXI BmgBI 2410 2420 2430 2440 2450 2460 2470 2480 2490 2500 AAAAGAGGCC CAGGAGTCCC AGTAGTCAGT CATCATCATC CGGGTCTCCA CCGCGCAGGC CCCCTCCAGG TAGAAGGCCA TTTTTCCACC CTGTAGGGGA BsaXI 2510 2520 2530 2540 2550 2560 2570 2580 2590 2600 AGCCGATTAT TTTGAATACC ACCAAGAAGG TGGCCCAGAT GGTGAGCCTG ACGTGCCCCC GGGAGCGATA GAGCAGGGCC CCGCAGATGA CCCAGGAGAA XcmI BmgBI BstXI 2610 2620 2630 2640 2650 2660 2670 2680 2690 2700 GGCCCAAGCA CTGGACCCCG GGGTCAGGGT GATGGAGGCA GGCGCAAAAA AGGAGGGTGG TTTGGAAAGC ATCGTGGTCA AGGAGGTTCC AACCCGAAAT AccB7I PflMI Van91I 2710 2720 2730 2740 2750 2760 2770 2780 2790 2800 TTGAGAACAT TGCAGAAGGT TTAAGAGCTC TCCTGGCTAG GAGTCACGTA GAAAGGACTA CCGACGAAGG AACTTGGGTC GCCGGTGTGT TCGTATATGG BsrDI EcoICRI SgrAI SacI Eco53kI 2810 2820 2830 2840 2850 2860 2870 2880 2890 2900 AGGTAGTAAG ACCTCCCTTT ACAACCTAAG GCGAGGAACT GCCCTTGCTA TTCCACAATG TCGTCTTACA CCATTGAGTC GTCTCCCCTT TGGAATGGCC AxyI BsmBI Eco81I Bsu36I 2910 2920 2930 2940 2950 2960 2970 2980 2990 3000 CCTGGACCCG GCCCACAACC TGGCCCGCTA AGGGAGTCCA TTGTCTGTTA TTTCATGGTC TTTTTACAAA CTCATATATT TGCTGAGGTT TTGAAGGATG PflMI BbvCI AccB7I Van91I 3010 3020 3030 3040 3050 3060 3070 3080 3090 3100 CGATTAAGGA CCTTGTTATG ACAAAGCCCG CTCCTACCTG CAATATCAGG GTGACTGTGT GCAGCTTTGA CGATGGAGTA GATTTGCCTC CCTGGTTTCC BsgI 3110 3120 3130 3140 3150 3160 3170 3180 3190 3200 ACCTATGGTG GAAGGGGCTG CCGCGGAGGG TGATGACGGA GATGACGGAG ATGAAGGAGG TGATGGAGAT GAGGGTGAGG AAGGGCAGGA GTGATGTAAC SacII 3210 3220 3230 3240 3250 3260 3270 3280 3290 3300 TTGTTAGGAG ACGCCCTCAA TCGTATTAAA AGCCGTGTAT TCCCCCGCAC TAAAGAATAA ATCCCCAGTA GACATCATGC GTGCTGTTGG TGTATTTCTG BsmBI AccI BalI MscI 3310 3320 3330 3340 3350 3360 3370 3380 3390 3400 GCCATCTGTC TTGTCACCAT TTTCGTCCTC CCAACATGGG GCAATTGGGC ATACCCATGT TGTCACGTCA CTCAGCTCCG CGCTCAACAC CTTCTCGCGT MfeI BmgBI MunI 3410 3420 3430 3440 3450 3460 3470 3480 3490 3500 TGGAAAACAT TAGCGACATT TACCTGGTGA GCAATCAGAC ATGCGACGGC TTTAGCCTGG CCTCCTTAAA TTCACCTAAG AATGGGAGCA ACCAGCAGGA SexAI 3510 3520 3530 3540 3550 3560 3570 3580 3590 3600 AAAGGACAAG CAGCGAAAAT TCACGCCCCC TTGGGAGGTG GCGGCATATG CAAAGGATAG CACTCCCACT CTACTACTGG GTATCATATG CTGACTGTAT XcmI 3610 3620 3630 3640 3650 3660 3670 3680 3690 3700 ATGCATGAGG ATAGCATATG CTACCCGGAT ACAGATTAGG ATAGCATATA CTACCCAGAT ATAGATTAGG ATAGCATATG CTACCCAGAT ATAGATTAGG NsiI EcoT22I 3710 3720 3730 3740 3750 3760 3770 3780 3790 3800 ATAGCCTATG CTACCCAGAT ATAAATTAGG ATAGCATATA CTACCCAGAT ATAGATTAGG ATAGCATATG CTACCCAGAT ATAGATTAGG ATAGCCTATG 3810 3820 3830 3840 3850 3860 3870 3880 3890 3900 CTACCCAGAT ATAGATTAGG ATAGCATATG CTACCCAGAT ATAGATTAGG ATAGCATATG CTATCCAGAT ATTTGGGTAG TATATGCTAC CCAGATATAA BstXI 3910 3920 3930 3940 3950 3960 3970 3980 3990 4000 ATTAGGATAG CATATACTAC CCTAATCTCT ATTAGGATAG CATATGCTAC CCGGATACAG ATTAGGATAG CATATACTAC CCAGATATAG ATTAGGATAG 4010 4020 4030 4040 4050 4060 4070 4080 4090 4100 CATATGCTAC CCAGATATAG ATTAGGATAG CCTATGCTAC CCAGATATAA ATTAGGATAG CATATACTAC CCAGATATAG ATTAGGATAG CATATGCTAC 4110 4120 4130 4140 4150 4160 4170 4180 4190 4200 CCAGATATAG ATTAGGATAG CCTATGCTAC CCAGATATAG ATTAGGATAG CATATGCTAT CCAGATATTT GGGTAGTATA TGCTACCCAT GGCAACATTA BstXI 4210 4220 4230 4240 4250 4260 4270 4280 4290 4300 GCCCACCGTG CTCTCAGCGA CCTCGTGAAT ATGAGGACCA ACAACCCTGT GCTTGGCGCT CAGGCGCAAG TGTGTGTAAT TTGTCCTCCA GATCGCAGCA BssSI 4310 4320 4330 4340 4350 4360 4370 4380 4390 4400 ATCGCGCCCC TATCTTGGCC CGCCCACCTA CTTATGCAGG TATTCCCCGG GGTGCCATTA GTGGTTTTGT GGGCAAGTGG TTTGACCGCA GTGGTTAGCG 4410 4420 4430 4440 4450 4460 4470 4480 4490 4500 GGGTTACAAT CAGCCAAGTT ATTACACCCT TATTTTACAG TCCAAAACCG CAGGGCGGCG TGTGGGGGCT GACGCGATCG CTGTTCCTTA GGACCCTTTT AsiSI AxyI SgfI Eco81I PvuI Bsu36I 4510 4520 4530 4540 4550 4560 4570 4580 4590 4600 ACTAACCCTA ATTCGATAGC ATATGCTTCC CGTTGGGTAA CATATGCTAT TGAATTAGGG TTAGTCTGGA TAGTATATAC TACTACCCGG GAAGCATATG 4610 4620 4630 4640 4650 4660 4670 4680 4690 4700 CTACCCGTTT AGGGTTAACA AGGGGGCCTT ATAAACACTA TTGCTAATGC CCTCTTGAGG GTCCGCTTAT CGGTAGCTAC ACAGGCCCCT CTGATTGACG HincII PsiI HpaI 4710 4720 4730 4740 4750 4760 4770 4780 4790 4800 TTGGTGTAGC CTCCCGTAGT CTTCCTGGGC CCCTGGGAGG TACATGTCCC CCAGCATTGG TGTAAGAGCT TCAGCCAAGA GTTACACATA AAGGCAATGT PciI BsrDI AflIII 4810 4820 4830 4840 4850 4860 4870 4880 4890 4900 TGTGTTGCAG TCCACAGACT GCAAAGTCTG CTCCAGGATG AAAGCCACTC AGTGTTGGCA AATGTGCACA TCCATTTATA AGGATGTCAA CTACAGTCAG DraIII ApaLI PsiI HincII Alw44I 4910 4920 4930 4940 4950 4960 4970 4980 4990 5000 AGAACCCCTT TGTGTTTGGT CCCCCCCCGT GTCACATGTG GAACAGGGCC CAGTTGGCAA GTTGTACCAA CCAACTGAAG GGATTACATG CACTGCCCCG AflIII BcgI PciI 5010 5020 5030 5040 5050 5060 5070 5080 5090 5100 CGAAGAAGGG GCAGAGATGT CGTAGTCAGG TTTAGTTCGT CCGGGGCGGG GATCTCGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG SpeI VspI AseI PshBI 5110 5120 5130 5140 5150 5160 5170 5180 5190 5200 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA ZraI AatII 5210 5220 5230 5240 5250 5260 5270 5280 5290 5300 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT AatII ZraI 5310 5320 5330 5340 5350 5360 5370 5380 5390 5400 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG AatII ZraI 5410 5420 5430 5440 5450 5460 5470 5480 5490 5500 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT Eco105I SnaBI 5510 5520 5530 5540 5550 5560 5570 5580 5590 5600 TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGGGC GCGCGCCAGG CGGGGCGGGG CGGGGCGAGG GGCGGGGCGG BsgI 5610 5620 5630 5640 5650 5660 5670 5680 5690 5700 GGCGAGGCGG AGAGGTGCGG CGGCAGCCAA TCAGAGCGGC GCGCTCCGAA AGTTTCCTTT TATGGCGAGG CGGCGGCGGC GGCGGCCCTA TAAAAAGCGA 5710 5720 5730 5740 5750 5760 5770 5780 5790 5800 AGCGCGCGGC GGGCGGGAGT CGCTGCGCGT TGCCCTCGCC CCGTGCCCCG CTCCGCCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC 5810 5820 5830 5840 5850 5860 5870 5880 5890 5900 TCCCACAGGT GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG CGCTTGGTTT AATGACGGCT TGTTTCTTTT CTGTGGCTGC GTGAAAGCCT AfeI Aor51HI Eco47III 5910 5920 5930 5940 5950 5960 5970 5980 5990 6000 TGAGGGGCTC CGGGAGGGCC CTTTGTGCGG GGGGAGCGGC TCGGGGGGTG CGTGCGTGTG TGTGTGCGTG GGGAGCGCCG CGTGCGGCTC CGCGCTGCCC 6010 6020 6030 6040 6050 6060 6070 6080 6090 6100 GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCAG TGTGCGCGAG GGGAGCGCGG CCGGGGGCGG TGCCCCGCGG TGCGGGGGGG AfeI BstZI SacII Aor51HI Eco52I Eco47III EagI 6110 6120 6130 6140 6150 6160 6170 6180 6190 6200 GCTGCGAGGG GAACAAAGGC TGCGTGCGGG GTGTGTGCGT GGGGGGGTGA GCAGGGGGTG TGGGCGCGTC GGTCGGGCTG CAACCCCCCC TGCACCCCCC BsgI 6210 6220 6230 6240 6250 6260 6270 6280 6290 6300 TCCCCGAGTT GCTGAGCACG GCCCGGCTTC GGGTGCGGGG CTCCGTACGG GGCGTGGCGC GGGGCTCGCC GTGCCGGGCG GGGGGTGGCG GCAGGTGGGG BlpI BsiWI Bpu1102I 6310 6320 6330 6340 6350 6360 6370 6380 6390 6400 GTGCCGGGCG GGGCGGGGCC GCCTCGGGCC GGGGAGGGCT CGGGGGAGGG GCGCGGCGGC CCCCGGAGCG CCGGCGGCTG TCGAGGCGCG GCGAGCCGCA SgrAI 6410 6420 6430 6440 6450 6460 6470 6480 6490 6500 GCCATTGCCT TTTATGGTAA TCGTGCGAGA GGGCGCAGGG ACTTCCTTTG TCCCAAATCT GTGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC BsrDI KasI EheI SfoI BbeI NarI 6510 6520 6530 6540 6550 6560 6570 6580 6590 6600 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG SfoI BbeI KasI EheI NarI 6610 6620 6630 6640 6650 6660 6670 6680 6690 6700 CCTCGGGGCT GTCCGCAGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT XbaI 6710 6720 6730 6740 6750 6760 6770 6780 6790 6800 AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTAACC CTCACTAAAG 6810 6820 6830 6840 6850 6860 6870 6880 6890 6900 GGGTACCGGG CCCCCCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTCCTGCA GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCGACTCTA Acc65I PspXI BanIIIHindIIIEcoRV EcoRI PstI BamHI SpeI XbaI NotI XbaI KpnI XhoI HincII BspDI Eco52I TliI SalI ClaI EagI PaeR7I AccI BstZI 6910 6920 6930 6940 6950 6960 6970 6980 6990 7000 GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT DraI BsaMI BsaBI BsmI MunI MfeI 7010 7020 7030 7040 7050 7060 7070 7080 7090 7100 GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG HincII PsiI BsaMI HpaI BsmI 7110 7120 7130 7140 7150 7160 7170 7180 7190 7200 GTTTGTCCAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA SspI 7210 7220 7230 7240 7250 7260 7270 7280 7290 7300 ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA PsiI BsaXI 7310 7320 7330 7340 7350 7360 7370 7380 7390 7400 GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC DraIII 7410 7420 7430 7440 7450 7460 7470 7480 7490 7500 CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG 7510 7520 7530 7540 7550 7560 7570 7580 7590 7600 GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC 7610 7620 7630 7640 7650 7660 7670 7680 7690 7700 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA BspHI 7710 7720 7730 7740 7750 7760 7770 7780 7790 7800 TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG SspI Eco81I PvuII AxyI Bsu36I 7810 7820 7830 7840 7850 7860 7870 7880 7890 7900 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA BbuI SexAI BbuI SphI SphI EcoT22I EcoT22I NsiI NsiI 7910 7920 7930 7940 7950 7960 7970 7980 7990 8000 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG NmeAIII 8010 8020 8030 8040 8050 8060 8070 8080 8090 8100 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCATCAAG AGACAGGATG AatI BsaBI NmeAIII StuI SfiI AvrII BlnI 8110 8120 8130 8140 8150 8160 8170 8180 8190 8200 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC EagI BstZI Eco52I 8210 8220 8230 8240 8250 8260 8270 8280 8290 8300 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG NarI EheI KasI SfoI BbeI 8310 8320 8330 8340 8350 8360 8370 8380 8390 8400 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA BalI FspI Tth111I MscI NsbI PflFI PvuII 8410 8420 8430 8440 8450 8460 8470 8480 8490 8500 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT NmeAIII BsrDI 8510 8520 8530 8540 8550 8560 8570 8580 8590 8600 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC SapI BspQI 8610 8620 8630 8640 8650 8660 8670 8680 8690 8700 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT BbuI SphI 8710 8720 8730 8740 8750 8760 8770 8780 8790 8800 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT CspI SapI RsrII BspQI CpoI 8810 8820 8830 8840 8850 8860 8870 8880 8890 8900 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT BssSI 8910 8920 8930 8940 8950 8960 8970 8980 8990 9000 TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG Csp45I BssSI BspT104I BstBI NspV 9010 9020 9030 9040 9050 9060 9070 9080 9090 9100 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA AvrII BlnI 9110 9120 9130 9140 9150 9160 9170 9180 9190 9200 CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT 9210 9220 9230 9240 9250 9260 9270 9280 9290 9300 TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG 9310 9320 9330 9340 9350 9360 9370 9380 9390 9400 GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AxyI DraI Eco81I Bsu36I 9410 9420 9430 9440 9450 9460 9470 9480 9490 9500 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA DraI BspHI 9510 9520 9530 9540 9550 9560 9570 9580 9590 9600 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 9610 9620 9630 9640 9650 9660 9670 9680 9690 9700 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 9710 9720 9730 9740 9750 9760 9770 9780 9790 9800 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 9810 9820 9830 9840 9850 9860 9870 9880 9890 9900 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG ApaLI Alw44I 9910 9920 9930 9940 9950 9960 9970 9980 9990 10000 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC BssSI 10010 10020 10030 10040 10050 10060 10070 10080 10090 10100 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 10110 10120 10130 10140 10150 10160 10170 10180 10190 10200 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC PciI AflIII 10210 10220 10230 10240 10250 10260 10270 10280 10290 10300 CGTATTACCG CCATGCATAG ATCT NsiI BglII EcoT22I